A high-resolution structural T1-weighted magnetization-prepared rapid gradient-echo (MPRAGE) MR image was taken for each patient using a 1.5T Siemens Sonata MRI scanner (sagittal plane; repetition time = 1970 ms; echo time = 4.38 ms; inversion time = 1100 ms; field of view: 512 × 512; in-plane voxel size: 0.5 × 0.5 mm2; slice thickness = 1 mm; 160 contiguous slices; flip angle = 15°).
DOI: http://dx.doi.org/10.7554/eLife.05421.017 Diffraction data collection using conventional x-ray sources typically employs the rotation method, in which a single crystal is rotated through a contiguous set of angles, and the diffraction patterns are recorded on a 2-D detector.
The functional volume was acquired for each participant with a T2*-weighted single-shot echo-planar imaging sequence (TR = 1,000 ms, TE = 30 ms, FoV = 224×224 mm, voxel size = 3.5×3.5×7.0 mm, 16 contiguous transverse slices, flip angle = 70°) sensitized to blood oxygenation level dependent (BOLD) contrast [24], [25].
A three-dimensional T1-weighted scan (Spoiled Gradient Echo; TE = 4.6 ms; TR = 30 ms; flip angle 30°; 160–180 contiguous coronal slices of 1.2 mm; in-plane resolution 1 × 1 mm; acquisition matrix 256 × 256) of the whole head was acquired of each subject.
Using these parameters, 43 contiguous slices were obtained angled parallel to the intercommissural line.
Functional data were aligned to high-resolution anatomical images obtained using a 3D T1 MPRAGE sequence (TE = 2.98 ms; TR = 2300 ms; TI = 900 ms; flip angle = 15°; 192 contiguous slices of 1.0 mm thickness; FOV = 192×256 mm2).
High-resolution T1-weighted three-dimensional MR images of the brain (magnetization-prepared rapid acquisition of gradient echo (MPRAGE) sequence: echo time = 3.47 ms, repetition time = 1930 ms, 1 mm3 isotropic resolution, flip angle 15°, 176 contiguous sagittal slices, matrix size 256 mm) were acquired at 3 Tesla (Magnetom, Siemens, Erlangen, Germany) with an 8-channel head coil.
The thresholds of the tracking termination were 0.2 for the FA and 45° for the angle between two contiguous eigenvectors.
During a single session, the following sequences were obtained: Axial dual-echo turbo-spin echo proton density and T2-weighted images (repetition time [TR] 2,600 ms, echo time [TE] 14/96 ms, flip angle 180°, 20 contiguous 5-mm thick axial slices, 200 × 512 matrix, field of view (FOV) 240 mm, two acquisitions, scan time 3 min and 34 s).
Each volume comprised 14 contiguous slices of 6mm thickness, angled at approximately 30 deg from axial to sample occipital, parietal, posterior temporal and posterior/superior frontal cortices.
Using these parameters, 20 whole brain contiguous axial sections were obtained angled parallel to the inter-commissural line.
