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For each combination of these categories, a model is presented and discussed in the context of the yeast cell cycle, illustrating how different types of questions can be addressed by different model classes.
Thus, both the FKBP12-rapamycin binding (FRB) domain and the kinase domain of mTor are fully functional in the context of the yeast cell [31].
These results illustrate that the human PP6-associated proteins are capable of providing distinct rapamycin-sensitive and Sit4-dependent Sap functions in the heterologous context of the yeast cell.
This experiment was carried out in the context of the yeast dual reporter vector pAC99, into which we had cloned the relevant BM2 information appropriately framed between β-galactosidase and firefly luciferase reporter genes.
In the context of the yeast mating response, there must be sufficient polarization to form a mating projection over a range of background pheromone concentrations, which may vary according to the number and proximity of mating partners, and at the same time, the ability to redirect the projection if the partner moves or mates with another cell.
Thus, it may prove both technically feasible and informative to express heterologous, multi-molecular signaling circuit ensembles in the context of the yeast cell as a proxy for their functions in the much more complex milieu of the multicellular eukaryotic system.
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b Oil content of the yeast strain.
We went on to investigate a number of these interactions by RNAi/DiGE analysis which has the power to provide context to the yeast two-hybrid data.
To this end, we adopted the R-SGA methodology (Kainth et al. 2009), which allows for high-throughput acquisition of the abundance of a protein of interest in the context of a yeast mutant collection.
In Figures 1B and C we mapped the positions of amino acids that are invariant among the canonical polymerases but different in NRPD1, NRPE1 or NRPD2/NRPE2 onto the S. cerevisiae Rpb1 and Rpb2 subunit structures in the context of a yeast Pol II elongation complex crystal structure.
These experiments showed that, at least in the context of a yeast nucleus, β1-syntrophin interacts robustly with all three α-dystrobrevin SBSs, and that the leucine-to-proline mutations ablate this interaction in each case.
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