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The first section describes the genomic context of the insertion.
The effect of point mutations in the context of the insertion of additional residues can be analyzed accordingly.
Hence the overall impact of a repeat insertion into an operon is difficult to predict, and is liable to change with the variation in the length of the BOX element and the context of the insertion site.
However, the un-induced level of d2eGFP varied considerably between clones, demonstrating some leakiness that most probably was dependent on vector copy number and the context of the insertion sites in each individual clone.
It is the most important single residue to determine specificity and activity within the context of the insertion and other aspects of apicomplexan LDH, but it is unhelpful to strongly analogize with the traditional specificity residue at #102.
Note that compared to these hits (designated TE3), the MER1A transposon in human ANRIL is located closer to the 3′ end of exon 3. To examine the context of the insertion of TE3, we used MEME, a program that detects potential motifs in multiple sequences, to analyse the exon 3 sequences.
Similar(52)
The location of the gene immediately upstream of its non-homologous, functional analogue suggests the genomic context of this insertion was decidedly non-random and points to the importance of context in the adaptation of a newly transferred gene.
3) Is there any commonality to the sequence context or genetic/epigenetic context of the RET insertion sites recovered?
Surprisingly, we did not observe any dependency of the expression pattern on the genomic context of the transposon insertion.
First, using the F84 model in PHYLIP, we calculated the pairwise distances of the concatenated rRNAs, exon 1, E3TE3, the left context of the TE3 insertion site, and exon 3 and of the concatenated rRNAs, exon 1, E8TE8a, and the ancient exon 8 (the 5′ end + E8TE8a) between human and other species.
Indeed the effect size might differ from study to study as a consequence of the different sample size, geometric features of the roundabout, the context of insertion, the driver population characteristics, and so on.
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