Specifically, we used the bioinformatic tool Phobius to predict the SP character of all possible combinations of 'mutations' in the context of the consensus sequence, and by calculating the ratio of the resulting predicted activity over the predicted activity for the consensus sequence.
To explore the fitness landscape more systematically, we have introduced the 64 combinations of mutations in the context of the consensus sequence, and evaluated their functional impact.
Within the context of this consensus statement, the focus of sub-group analysis is on baseline factors that moderate treatment effects.
A very telling example of compensatory mutations is provided by the mutations K6N+F11V+P20L (i.e. mutations 1, 3 and 4 placed in the context of the consensus sequence).
14-3-3 14-3-3 14-3-3 14-3-3indimerswo pSer/pThr residues within the classicallysurrounding consensus amino acid residues (Obind and Obsilova, 2011).
The 'mutation' C12Y (in the context of the consensus), is predicted to be deleterious when present alone, as it drives down the SP activity to 51% of that of the consensus.
For instance, combinations involving mutations 1 and 4 together (in the context of the consensus) are highly problematic, but their effects are compensated by mutation 5. Interestingly, some mutations appear rather 'hypermorphic' with respect to the consensus.
Despite constant levels of ER expression, ER transcriptional activity was lower in MCF-7TamR cells than in control cells in the context of a consensus oestrogen response element (Fig 1E).
The archetypical member of this family of proteins is Kaiso, a member of the BTB/POZ (POZ/ZF) zinc finger domain family of proteins that binds methylated CpGs in the context of a consensus sequence composed by at least two consecutive methylated CpG dinucleotides.
Moreover, the upstream start codon at position 6,539 of the peach isolate is in the context of Kozak consensus sequence requirements (A at position −3 and G at +4) for translation initiation [ 2, 8], which are not satisfied by the corresponding upstream start codon in the cherry isolate sequence or by the downstream start codons of either isolate.
