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In this study, we evaluated codon context fitness as design criterion for improving the expression of thermostable invertase from Thermotoga maritima in Escherichia coli and explored the relevance of secondary structure regions for folding and expression.
From the resultant enzyme activities and protein yield data, codon context fitness proved to have the highest activity as compared to the wild-type control and other criteria while secondary structure-based strategy is comparable to the control.
In this evolutionary context, fitness measures the expected reproductive success of an individual having a specific genotype in some particular environment.
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In different contexts, fitness, yield or productivity could be used.
Rather, it is antigenic evolution in the context of fitness variation generated by deleterious mutations that constrains the viral phylogeny.
Energy minimization in biological operations is not a new hypothesis, especially in the context of fitness and adaptation to the environment [ 20- 22].
We have performed follow-up experiments that validate conditional regulation by two FFRPs, and demonstrate a context dependent fitness benefit for the regulation.
In an evolutionary context, reproductive fitness is commonly used to estimate selection, as in studies investigating crop allele persistence in wild populations [ 1- 3].
However, we believe that more work focussed on these root responsive strategies is needed, particularly in the context of fitness consequences, competitive tolerance and avoidance, community assembly and ecosystem function.
In a selection context where fitness is attained by females that wean large litters in short intervals, selection of line V seems to favour females that can rapidly change the priority between the current (kits being nursed) and future litters (foetus development).
The latter monitors users' positions and other physiological parameters in outdoor sports activities to present functionalities such as location-aware maps augmented with visualizations of users' performance, or context-aware fitness advice and 3D demonstrations of exercises.
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