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The contents were mixed and poured onto glucose minimal agar plates immediately.
The contents were mixed on shaker for 30 min at 300×g.
The contents were mixed well and filtered rejecting the first portion of the filtrate.
The contents were mixed well and filtered; the first portion of the filtrate was rejected.
Five to ten microliters of 20% SDS were added, and the contents were mixed until they became clear visible.
To this, the biosynthesized nanoparticles dispersion solution (0.3 ml) was added and the contents were mixed well.
The reaction was then cooled in an ice bath and the contents were mixed with approximately 3 mL of a 1 1 (v/v) mixture of water:cyclohexane.
The final contents were mixed using a spatula, and again a final mixing was performed using the ACE NISSEI homogenizer at 5000 RPM for 15 30 min to get the homogenous electrode slurry.
The reaction was initiated by the addition of arachidonic acid dissolved in DMSO (50 µL, 100 µM in the assay) to the enzyme/extract mixture and contents were mixed immediately.
The composites with different SWCNTs (0, 1, 2, 3 phc) and maleic anhydride grafted polyethylene (MAPE) (0 and 3 phc) contents were mixed by melt compounding in an internal mixer and then the composites manufactured by injection molding method.
As there was no free flowing water in SSF, after microbial growth the contents were mixed with 40 mL of citrate buffer (50 mM, pH 5.0) and then incubated in an orbital shaker for 60 min at 200 rpm such that the loosely bound proteins will be solubilized into the extracellular culture extract.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com