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The samples with about 100 micrograms of total protein content were dissolved in 50 μL of saline and intradermally inoculated in the dorsum of anesthetized mice with ethyl ether.
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The siliceous oozes exist only where the rate of deposition of diatoms or radiolarians is greater than the rate at which their silica content is dissolved in the deep waters; thus the diatom oozes are confined to belts in the North Pacific and Antarctic, and the radiolarian oozes are found only under the eastern part of the North Pacific.
This content was dissolved in sterile saline (0.9 % w/v NaCl) to obtain a solution of 2%% (w/v) concentration (Tam et al. 2011).
Lactobacillus capsules were swallowed as such, or the capsule content was dissolved in cold milk or juice.
The contents were dissolved by repeated vortexing.
The contents were dissolved in water, vortexed, and centrifuged thereafter at 3,000 g.
When the active ingredients were insoluble in water (nelfinavir and saquinavir), the contents were dissolved in dimethylsulphoxide (DMSO); care was taken to ensure that the final concentration of DMSO in the dilutions would not exceed 1%.
Biliary contents were dissolved and diluted in methanol (Burdick & Jackson, Muskegon, MI, USA) and analyzed using ESI/MS/MS on a Hewlett-Packard HP 1100 MSD operated in the negative mode.
In eight of the nine studies involving mechanically ventilated patients (Table 3), probiotic powder or capsule contents were dissolved in water and delivered via a feeding tube into the stomach.
After evacuating and refilling the flask with nitrogen three times, dry toluene (total toluene volume 7.526 mL, including CPETC solution) and dry acetonitrile (5.211 mmol, 0.272 mL) were added to maintain a monomer concentration of 0.67 M. The mixture was stirred at room temperature until contents were dissolved.
To determine gadolinium content, samples were dissolved in 1% nitric acid, and the amount of gadolinium in the solution was determined by inductively coupled plasma emission spectrometer (ICP-AES, iCAP 6,000 Series, Thermo Scientific, USA).
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