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Measurements of grain size, magnetic susceptibility (MS), total nitrogen (TN), total organic carbon (TOC), total inorganic carbon (TIC), atomic TOC/TN (C/N) and carbonate content were conducted in order to reconstruct the climate since ∼3700 cal a BP.
The base farm did not have a high probability of success; so, a sensitivity analysis for reductions in CAPEX and OPEX and increases in biomass production and lipid content were conducted.
Measurements of phenolic content were conducted on the same samples as the expression analysis.
The tests for heavy metal and microbial content were conducted by Tianjian Pharmaceuticoal Ltd.
Statistical analysis of wet weight and water content were conducted using a two-way ANOVA with a Tukey's Honestly Significant Difference post hoc test, p < 0.05 (SAS 9.1, SAS Institute Inc. Cary, NC).
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Determination of total tannin content was conducted using a similar method for the determination of TPC described by Makkar et al. [18].
The measurement of total phenolic content was conducted by the method as described by Quettier-Deleu et al. [36], with some modifications.
Field research testing the effectiveness of each episode's content was conducted at preschools, prior to broadcast.
Quantification of DNA content was conducted by an adaptation of previously reported methods [36,37].
To determine the ploidy and confirm the haploid form, flow cytometry for nuclear content was conducted.
Critical revision of the manuscript for important intellectual content was conducted by OB, PH, AE and TM.
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