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The total silver concentration content was quantified by ICP-MS.
TP content was quantified according to the method described by Singleton et al.[39].[39]
The total phenolic content was quantified according to the method of Singleton et al. [37].
Subsequently, their heterologous protein content was quantified to determine if selenocystamine had an effect on protein activity.
To determine which fractions were the enriched iISGs or mISGs, the insulin content was quantified using an ELISA assay.
The intracellular Cd2+ content was quantified using inductively coupled plasma-mass spectrometry (ICP-MS) and compared with standards.
Also, the gold content was quantified by inductively coupled plasma mass spectrometry (ICP-MS) and neutron activation analysis (NAA).
Collagen content was quantified as total insoluble hydroxyproline on day 5. Zymography and immunoblotting were used to detect matrix metalloproteinases.
Protein content was quantified using EZQuant (Invitrogen).
Total protein content was quantified (NanoDrop).
Protein content was quantified using the micro Bradford assay [45].
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