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Protein content was estimated by the Bradford method37.
The unfreezable water content was estimated as 36.7%.
The carbohydrate content was estimated by the phenol-sulfuric method using glucose (Glc) as standard52.
Mitochondrial content was estimated by means of mitochondrial yield and citrate synthase activity.
The As V) content was estimated by difference between both measurements.
The femoral bone mineral content was estimated using inductively-coupled-plasma mass spectrometry.
Radioactivity and protein content was estimated.
OH content was estimated below 1 wt ppm.
Then brain blood content was estimated by hemoglobin ELISA.
The protein content was estimated using the Bradford method.
Finally, protein content was estimated by the differences.
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