This gene signature, hence forth called the 'vascular content' signature, is presented in Fig. 1B.
The highest expression of the vascular content signature was observed in claudin-low mouse tumors (Fig. 1B).
Interestingly, only two genes from our 74-gene vascular content signature overlapped with the 58 genes from Wallgard et al. (EGFL7 and ESAM).
This signature was distinct from the previous two vascular profiles as determined by gene overlap, with one gene overlapping with the Wallgard et al. signature (SLCO2A1) and one gene overlapping with the vascular content signature (CYP1A1).
We propose that the reason the normal breast samples have relatively higher levels of both the Wallgard et al. and vascular content signature are due to the relatively high endothelial content found in a normal breast.
For the vascular content signature, RNA was prepared from human breast cancer cell lines (MCF7, T47D, SKBR3, SUM149, SUM159, MDA231), endothelial cells (HUVEC, LEC, BEC) and commercial RNA for human tissue was obtained from Ambion (brain; AM6050), (lung; AM7968), (liver; AM7960), (lymph node; AM7894), Clontech (bone marrow; 636591), and Biochain (bone marrow; R1234024-10).
This approach can enable message sender to generate source anonymous message signature with content authenticity assurance, while hiding the real identity of the message sender.
Figure 3 also showed that in the presence of peanut oil, the content of signature peptide was about 40% of the signature peptide in the standard mix.
We show that elevated CpG content, a signature of historically weak germline methylation, is linked with environmentally driven gene expression.
