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As a step towards the goal of increasing beta-cell function, proliferation or survival, we designed a multi-parameter, high content screening platform that enabled us to screen a library containing 1319 unique natural marine invertebrate extracts.
Intensity of 53BP1 was measured using the Thermo Scientific Cellomics high content screening platform where the intensity of individual foci per nucleus was assessed.
The high content screening platform described here also enables chemical genetics strategies for the identification of novel cellular pathways and targets impinging on p27 - for example, the observation that SMIP004 strongly downregulates SKP2 appears significant with regards to its mechanism of action.
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This method could be scaled for use with higher throughput assaying via the FLIPR, or even for integration with high content screening platforms.
Image acquisition and counting of various fluorophore-stained entities were accomplished with a high-content screening platform.
Further work will be required to increase the lentiviral infection rate and beta-cell purity before this high-content screening platform can be routinely applied to human islet cultures.
Other companies offer similar tools on their own high-content screening platforms, such as GE Healthcare Life Sciences' IN Cell Analyzer system.
The higher throughput represents a significant improvement over existing high-content screening platforms.
Slides were imaged using the CellInsight CX5 High Content Screening (HCS) Platform (Thermo Fisher Scientific).
A new high-content screening software platform enables cancer researchers to uncover more about basic and advanced cellular function to accelerate cell-based research.
Klein, O. J., Bhayana, B., Park, Y. J. & Evans, C. L. In vitro optimization of EtNBS-PDT against hypoxic tumor environments with a tiered, high-content, 3D model optical screening platform.
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