In addition, probe T m, GC content, probe position on the original RefSeq sequence and ΔG of the most stable probe secondary structure calculated using MFOLD-software (Zuker et al., 2003).
We used as predictor variables several sequence-derived probe characteristics, including Tm, probe length, probe GC content, probe purine content, and the presence of a known SNP in the probe.
This group found that probe uniqueness, SNP content, probe length, Tm, and guanine homopolymers all influenced probe performance[24].
Furthermore, coverage is influenced by array design, including probe sequence (melting temperature, GC content), probe density and spacing (Supplementary Table 1).
However, calling SNP based on hybridization signals is a complex process, and many factors can affect SNP calling, including array design, GC content of a probe, the position of the SNP in a probe, hybridization conditions, and algorithms used to analyze array signals.
We show that rimantadine, an inhibitor of M2 proton conductance, blocks the acidification-dependent dissipation of fluorescence from a pH-sensitive virus-content probe.
Electrical measurements were performed under different electric fields and over time on each filler content, in order to probe the particles structuration.
We can, however, with appropriately designed experiments and tests probe the content of what the rat has learned and the psychological constructs guiding its behavior.
These insects, which harbor a γ-proteobacterial symbiont named Ishikawaella capsulata in their midgut crypts, had been known for their unique mechanism for vertical transmission, so-called 'capsule transmission' [ 24, 25], wherein mother insects deposit symbiont-filled particles (symbiont capsules) in association with eggs, and hatchlings probe the content of the capsules to acquire the symbiont.
These high-GC-content probes are therefore likely from the var genes.
