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The traditional assay used to measure potency of inactivated influenza vaccines is a single-radial immunodiffusion (SRID) assay that utilizes an influenza strain-specific antibody to measure the content of virus hemagglutinin (HA) in the vaccine in comparison to a homologous HA reference antigen.
The HEV RNA content of virus samples were determined prior to inoculation by reverse transcription-polymerase chain reaction (RT-PCR), as described previously [20], [23].
The genome content of virus stocks was quantitated by real-time PCR (Rotor-Gene, Corbett Research) of the MuHV-4 M2 gene (forward primer genomic co-ordinates 4166-4188, reverse primer 4252-4228, Taqman probe 4219-4189).
The receptor-cleavage function of newly synthesized HN seems to play an essential role with regard to the HN content of virus particles and consequently to the infectivity of viral progeny.
Earlier results revealed that growth of a genetically modified HN-negative mutant was unimpaired by the presence of HN protein and that the HN content of virus particles was drastically reduced when infections with the NA-deficient Sendai virus mutant ts 271 were performed at 39°C [ 17, 29, 35].
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The genome sizes (defined as haploid DNA content) of viruses vary greatly between species.
So, there is a strong pressure on the genome content of viruses with small capsids leading to the loss of genetic information unnecessary for virus propagation.
One hypothesis to explain this discrepancy relies on distinct viral DNA contents of virus stocks.
Difference in sensitivity and specificity between culture and nPCR and also the analysis of the impact of cell content on virus recovery in patients with clinical genital herpes infection versus those without evidence of HSV infection was determined by a two-tailed Chi-square test.
Ms. McClain, for example, claimed she encountered many difficulties in her attempts to learn the genetic content of the virus she suspected had infected her because it was protected as a trade secret.
To develop a strategy utilizing a number of biochemical methods for the characterization of the viral growth properties and protein yield in eggs, we have selected eight H5N1 pre-pandemic viruses and determined the viral Egg Infectious Dose 50 (EID50), total protein yield, hemagglutinin (HA) to nucleoprotein (NP) ratios (HA NP), and HA1 content of each virus.
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