Sentence examples for content of the restriction from inspiring English sources

Matching the GC content of the restriction enzymes with that of the target cDNA is a relatively simple step to optimize experimental design.

The number of AFLP fragments is normally decreased (although, for some species, increased) with the GC content of the restriction sites (2/10 GC nucleotides for EcoRI/ MseI, 5/10 for BsmI/ TaqI, and 8/10 GC for SacI/ HpaII).

Note that the percentage of AFLP fragments inside gene sequences is increased with an increase of the GC content of the restriction sites for all cases except for Oryza.

Since IS4, B3 and UK4 VL sequences differ in their content of the restriction sites Aat II and Ava I, we checked that the desired parts of each sequence were present in the new hybrid sequences by carrying out Aat II, Hind III/ Ava I and Aat II/ Bam HI digests.

This difference is likely caused by the length and GC content of the restriction sites that were targeted in the digestion of the genome: both the HindIII and EcoRI sites (AAGCTT and GAATTC, respectively) have a length of six nucleotides and a GC content of 33.3%, whereas the MboI site (GATC) has a length of four nucleotides and a GC content of 50%.

Furthermore, since the libraries of each species were constructed with two restriction enzymes (EcoRI and BamHI) complementary in the GC content of their restriction sites, the genome coverage should be much better distributed along the genome than those constructed with a single enzyme [ 13, 36].

That is the message of the restrictions.

The swelling ratio of nanofibers decreased with increasing the MWCNT content due to the restriction of swelling in the presence of MWNTs.

Matching the GC content of a restriction site to a genome can also be used to influence marker number.

"Would it support restrictions on content of the product?

Central to this project, we wanted to make the content of the platform open to any investigator without restriction.