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Adhesion of Ni films evaluated by the Scotch® tape test mainly depends on the phosphorus content of the plating bath used, i.e. on the amount of stresses generated in the film which depends on the quantity of phosphorus present, the latter originating from the plating bath reducer.
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The source and content of the plates both do and don't matter to her, it seems.
An analysis was also carried out to determine the moisture transfer coefficients that vary with the moisture content of the plate during drying.
Figure 4a shows the results for the changes in the average dimensionless moisture content of the plate (ϕ avg) during the drying process which can be defined as Fig. 4 Variation of the dimensionless average moisture content in the SiC plate with respect to drying time.
The content of the plate was then decanted and 50 ml of crystal violet added to each well.
Media content of the plates was replaced daily during the treatment, and percent changes in the tetanic forces relative to the vehicle control were recorded.
After each step, the content of the plate was removed, the plate was washed, and each well was filled with a specific reagent; the plates were then incubated for 30 min.
After 45 min of incubation at room temperature, the content of the plate was decanted and the plate was rinsed ten times in DI water by alternate submerging and decanting.
After this, the contents of the plates were replaced with 50 μL DMSO, and optical density at 540 nm was measured after background correction at 690 nm using the Spectramax 190 microplate spectrometer.
After this incubation, the contents of the plate were discarded and the plate was rinsed 5 times with wash buffer supplied by the manufacturer.
Subsequently, the entire contents of the plates were collected and lyophilized for 48 hours.
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