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The endocytic compartments were collected using a magnetic separator (Invitrogen Life Technologies, Grand Island, NY, USA) and drug content of the compartments was determined by HPLC.
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Prior to the dissolution experiments, the contents of the compartments were heated to 37°C.
The droplets are evaporated by multipass laser beam in an IR-reflective electrostatic tube, the content of the aqueous compartments is ionized, and the ions are transferred to the high-vacuum region and mass analyzed in a reflectron time-of-flight mass spectrometer.
The C-sucrose specific radioactivity is the C-radioactivity recovered as sucrose from a compartment divided by sucrose content of the same compartment.
In contrast, if the ACh release were inside a striosome, the low AChE content of this compartment could facilitate ACh activation of the same receptors (Fig. 6B).
(6) Given that the total osmolar content of the intracellular compartment is twice as high as that of the extracellular compartment, the total body water is divided accordingly.
(11) Hence, the intracellular osmotic content is slow to adapt when the sodium and water content of the extracellular compartment is disturbed.
Exposure to a warm environment for 30 minutes did influence the heat content of the peripheral compartment and reduce the core-to-peripheral flow of heat.
From these values and by application of the steady-state assumption all iron fluxes can be estimated when the iron content of the central compartment is available.
The pools accessible for radioactive labels (Additional file 1: Table S10) may be compared with the non-heme iron content of the same compartment (Additional file 1: Table S12).
Thus, the accumulation of ascorbate in the thylakoid lumen of plants exposed to high light conditions and the general increase inside the stroma highlights the importance of high ascorbate contents for the compartment specific protection of chloroplasts during high light conditions.
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