Assuming no data caching and no special connection, the proxy may deploy attacks on location verification process if it is able to learn about data accesses and to classify them into two categories: i) data accesses used only for location verification process and the content of read data is not relevant, only RTTs matter, and ii) data accesses where content is relevant but no RTTs are collected.
The GC content of reads produced by MinION™ showed an important and significant correlation (Pearson's r = 0.47, p ≤ 0.0376) against the GC content of reference values (Fig. 3a), which indicates that the GC content of 16S rDNA sequences is fairly well replicated during sequencing.
On the other hand, we found a strong correlation between the GC content of reads and the mismatch rate retrieved from alignments, which would insinuate again that GC content is a factor that influences 16S rDNA amplicon sequencing in the MinION™ platform.
These differences may affect the GC content of reads (Additional file 6: Table S4) and the coverage of specific loci, which can influence variant calling, although the global coverage was similar at the level of 10× sequencing depth (Table 1, Additional file 1: Figure S1).
To further investigate the effect of GC content on reads coverage, we examined the hidden relationship between chromosome structure and inherent GC content.
We first ran five different SeqPig read statistics for a different number of computing nodes: the sample distribution of (i) the average base quality of the reads; (ii) the length of reads; (iii) bases by position inside the reads; and (iv) the GC contents of reads.
This was done to assure equal time on task by providing both the control and experimental groups with relatively the same amount of content to read and comprehend.
They were not content to read accounts of parliamentary debates and analyses of British diplomatic endeavour.
Although at first Siegfried discussed his festival plans with her, she avoided the Festpielhaus, content to read reports of the productions.
"There's always another press release or journal table of contents to read that you don't get to," he says.
Facebook page owners can curate who can participate in terms of reading content and posting comments and reactions.
