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Drug quality was assessed at our laboratory in London using the GPHF MiniLab® as the screening tool, HPLC photodiode array detection (PDA) to measure content of formulations and the in vitro dissolution testing adhering to USP monographs to decide on the bioavailability of the antibiotic samples.
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Even for the PW formulations, simulating the increase in moisture content of lyophilised formulations upon storage, no decrease in SC content could be observed with the exception of the sucrose-based formulation.
Content of the formulations of amoxicillin and co-trimoxazole was measured by dissolving each formulation in solvent (0.1 M HCl for amoxicillin and methanol for co-trimoxazole) to give a 1 mg/mL solution that was further diluted to obtain a 0.5 mg/mL solution, and analyzed using our in-house HPLC method outlined above.
The median size of the pellets of the "good formulation" could only be related to the chitosan and sodium alginate content of the formulations.
However the study evidenced that sodium alginate and Acrycoat E30D do not have much significant effect in the drug content of the formulations, since the drug contents were within ±5% of the labelled potency of the drug in all the formulations.
The dry matter (DM) content of the formulations ranged between 20.1 % for Ryzoset and 62.2 % for Manek, and in contrast, ash contents of the formulations were between 1.5 % (Rygex) and 15.4 % (Algavyt Zn/Mn) (Table 2).
This work deals with Product Design by means of theoretical predictions of the Solid Fat Content of different formulations using 3 vegetable oils.
In summary, it was shown that with proper design, even major differences in the total drug content of IVR formulations might not result in significant effects in the in vitro and in vivo release properties.
Uniformity in thickness and drug content of all formulations are shown in Table 2.
The carbohydrate content of the formulations was measured by anthrone method [28].
The EAAI is a rapid method to evaluate and optimize the amino acid content of food formulations (Smith and Nielsen 2010).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com