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130 µg (protein content) of extract was incubated with 2 pmol of biotinylated RNAs, and streptavidin captured RNA-protein complexes were resolved on a 10% SDS polyacrylamid gel.
Total phenolic content of extract was measured using a modified Folin-Ciocalteu procedure [ 25].
Erkan et al. [ 35] reported a close correlation between radical scavenging activity and total phenolic content of extract from various natural sources.
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In this article a combined classifier based on the information content of extracted features from the primary structure of protein has been introduced to face this challenging problem.
Protein content of extracted samples was measured with Bradford's reagent.
To evaluate the DNA content of extracted chromosomal DNA preparations, detection of human β-globin gene was carried-out by PCR, as described previously [71].
The components and content of extracted lipids were determined by modified thin-layer chromatography (TLC) method 19.
Protein levels were normalized to ng/μg total protein content of extracted mucosal samples or ng/ml for sera.
A response surface methodology has then been used to maximize total polyphenol content of extracts and investigate influence of parameters involved in extraction procedures for both total polyphenols content and composition of extracts.
The total flavonoid content of the extract and fractions was expressed as quercetin equivalents (QE) mg/g of dry extract.
The average phenol content of the extract was 46.44 ± 1.022 mg Gallic acid equivalent per gram of the plant extract.
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