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However, given a protein with a high content of charged residues, a variety of electrostatic and hydrophobic interactions are involved in the liquid solid interface between the protein and nanopore [31].
All of the VP3 sequences contained a low content of charged amino acids (less than 24.87%) and a high content of hydrophobic and non-polar residues.
The high PONDR scores associated with most of the OPN sequence should come as no surprise, since it has all the hallmarks of an intrinsically unordered protein: a high content of charged amino acids, a low content of hydrophobic amino acids and a high degree of sequence redundancy [69].
The implication is that a rigid orientation of a peptide in chromatography confers sensitivity to its sequence as well as to the total content of charged residues.
This trend indicates the correspondingly decreased content of charged residues, increased content of structured-promoting residues, or a combination of these two factors.
HMG proteins all have an unusual amino acid composition characterized by a high content of charged amino acids and a high content of proline [ 3].
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The sequences varied in length from 293 to 485 amino acids and they showed very different contents of charged amino acids and theoretical isoelectric points (pI) ranging from 5.17 to 9.66 (Additional file 4).
As the relative sp2 content of the charged film is believed to be the governing factor toward effective charging [21], local accumulation of very small grains under the surface on the specific area being charged may lead to an increase in the local sp2 content (more grain boundaries).
Benefiting from the high electrical resistivity of polyvinyl butyral (PVB) and high dielectric constant of silicon nitride nanoparticles (Si3N4 NPs), PVB/Si3N4 nanofibrous membranes are endowed with improved stable surface potentials from 1610 to 2010 V when the content of charge enhancer (Si3N4) increases from 0too 1 wt%.
Note their wide distribution in the plot, their high content of charges residues and low hydrophobicity.
Binding sites for polycations are already known in the vasculature of the lungs, and we postulated that these could also be involved in histone accumulation, since histones have a high content of positively charged amino acids.
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