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As a result, the proportion of sequence reads mapped successfully from a given read pool ranged from over 90% to under 20%, which in turn would result in up to ca. five-fold under-estimation of the endogenous content of a read pool.
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On the example of 454 reads with an error rate of 0.49%, we further investigated to which extent the GC-content of a read influences gene prediction accuracy.
To assess the overall comparability of the pyrosequencing datasets obtained from the GS FLX and Titanium platforms, the average GC content of all reads was determined for each dataset.
The 54-year-old Eta militant is wanted in connection with the contents of a letter read out at a rally in San Sebastián the day after his release in August 2008.
When you read a biology textbook, the content of what you read is what matters.
These are triggered by tags, the content of which is read as a keystroke.
The overall GC content of the reads is 42%.
We found no significant difference in the GC content of unmapped reads between the methods (Additional file 5: Figure S1).
The reason behind the differences in assembly results may be the sequence content of the reads, the real covered regions in the genome or other factors.
The rRNA analysis files downloaded from the RDP website were then imported into the software MEGAN [ 71] to analyze the taxonomic content of DNA reads.
The GC content of mRNA reads was not appreciably affected by PDD (<2% difference), unlike previously reported with C0T hybridization [ 20].
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com