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Strikingly, the content mixing histogram showed more pronounced short-time scale content mixing upon Ca2+ injection than in the absence of complexin.
However, placing PI3P in the R-SNARE liposomes, vacuolar SNAREs alone can also induce efficient content mixing without HOPS.
Likewise, at 250 μM Ca the effect of complexin is more pronounced on the content mixing histogram compared to the lipid mixing histogram (compare Figures 6b and 8b).
The Nunnari laboratory has already optimized confocal and EM approaches that stage the reaction at each step of docking, outer membrane fusion, inner membrane fusion and matrix content mixing [ 5- 7].
Content mixing will start like that, with audio and video mixed under the same corporate umbrella.
Also, the similarity in rate constants for lipid mixing and content mixing found here for HA-mediated fusion and by Lee and Lentz (Proc. Natl. Acad. Sci.
This model includes the measurable steps in the fusion process, i.e., first pore formation, lipid mixing, and content mixing of aqueous fluorescent markers.
Comparison of lipid mixing and content mixing assays.
Most importantly, a true content mixing assay was used.
(A ) Kinetics of 1R-3Q content mixing assays.
3) The content mixing assay also shows asymmetry.
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