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So far no one has successfully brought Internet video to the big screen in a way that satisfies consumers and content markers.
We provide new information concerning the effect of inhibiting intestinal cholesterol absorption with ezetimibe in addition to weight loss alone on intrahepatic triglyceride content, markers of inflammation, and apoB-100 metabolism in subjects with obesity.
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Acrosomal status was determined separately, using the acrosome content marker Pisum sativum agglutinin, linked to fluorescein isothiocyanate (FITC-PSA).
The membrane was probed with antibodies against cathepsin D (azurophilic granule content marker) and GM130 (Golgi marker, used as a negative control).
We therefore assessed the fate of the endolysosomal content marker dextran-10 kDa over the course of M. tuberculosis infection.
Sperm viability and acrosomal integrity were assessed using the LIVE/DEAD sperm vitality kit and the acrosomal content marker PSA-FITC, respectively.
Acrosomal integrity was evaluated using the acrosomal content marker Pisum Sativum agglutinin coupled to fluorescein isothiocyanate (PSA-FITC), as described elsewhere (Mota et al., 2012).
Sperm acrosome integrity and induction of apoptosis were assessed using the acrosomal content marker PSA-FITC and annexin V kit, respectively.
5. It would be nice (but not required) to investigate the effect of the same mutations on content mixing using the content marker that was used in the previous PNAS study.
Genome-wide association studies (GWAS) using fixed content marker arrays represent a genotype sampling strategy that has been successfully used in a number of studies to identify SNPs significantly associated with complex traits [ 1- 3].
Citrate synthase activity, which is largely used as a mitochondrial content marker, was found to be ∼2-fold higher in cold-acclimated fish than warm-acclimated fish (0.44±0.02 vs 0.23±0.02 µmol citrate mg−1 minute−1, n = 5 fish per group; P<0.0001), suggesting that there is a higher mitochondrial content after cold acclimation.
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