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Acrosomal status was determined separately, using the acrosome content marker Pisum sativum agglutinin, linked to fluorescein isothiocyanate (FITC-PSA).
The membrane was probed with antibodies against cathepsin D (azurophilic granule content marker) and GM130 (Golgi marker, used as a negative control).
We therefore assessed the fate of the endolysosomal content marker dextran-10 kDa over the course of M. tuberculosis infection.
Sperm viability and acrosomal integrity were assessed using the LIVE/DEAD sperm vitality kit and the acrosomal content marker PSA-FITC, respectively.
Acrosomal integrity was evaluated using the acrosomal content marker Pisum Sativum agglutinin coupled to fluorescein isothiocyanate (PSA-FITC), as described elsewhere (Mota et al., 2012).
Sperm acrosome integrity and induction of apoptosis were assessed using the acrosomal content marker PSA-FITC and annexin V kit, respectively.
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So far no one has successfully brought Internet video to the big screen in a way that satisfies consumers and content markers.
We provide new information concerning the effect of inhibiting intestinal cholesterol absorption with ezetimibe in addition to weight loss alone on intrahepatic triglyceride content, markers of inflammation, and apoB-100 metabolism in subjects with obesity.
The use of fluorescent stains (enzyme-reactive substrates conjugated to fluorochromes) can increase signal sensitivity and dynamic range of detection and can also enable a variety of multiplexing applications for high-content marker analysis [ 16,81,93 95,101].
Overall, combining multiparametric flow cytometry with high-content markers for autolysosomal degradation pathways improves standard screening methods [ 14- 16] due to reduced risk of potential off-target effects by the addition of lysosomal inhibitors.
where PIC i is the polymorphism information content of marker i, f i is the frequency of the marker fragments which were present and 1 − f i is the frequency of marker fragments which were absent.
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CEO of Professional Science Editing for Scientists @ prosciediting.com