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For uniform and standard quantification of biomarkers in plant samples, it is necessary to remove the moisture content in the test sample.
Sample solutions were collected periodically and analyzed in the AAS for the metal content in the test solution after adsorption; the procedure was continued till the equilibrium conditions were obtained.
Tests were designed to allow for additional testing of microbial activity (dehydrogenase activity (DHA)), potential nitrification and supply of plant-available nitrogen (NO3-N content) in the test substrates.
The final concentration of pomegranate extract ranged from 0.097 – 12.5 mg/ml, reducing the methanol content in the test extract to between 0.19 and 20%, whereas streptomycin was between 0.78 and 100 μg/ml.
The nugget effect may result in the variation in toxin content in the test sample and for that reason the recommended procedure for OTA analysis includes two grinding steps.
The calibration curve was prepared with quercetin (20 200 μg) and the flavonoid content in the test samples were expressed as μg quercetin equivalent/mg sample (μg QE/mg).
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Samples were taken after 20 days for estimation of cell count and phosphorus content in the tested algal cells.
In case of the FRAP method, the Fe2+ content in the tested samples of homogenate brain and plasma was calculated based on the standard curve.
The contents in the test tubes were then incubated overnight at 37 °C.
B and b expressed the number of his+ in the new negative controls - histidine content presented in the test plates corresponded to the free histidine content of SAA (columns B) and total histidine (columns b), respectively.
This was done to maintain content coherence in the test.
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