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An objective method of interpreting cytopathic changes associated with cervical disease, known as DNA ploidy analysis, involves the numerical measurement of DNA content in the nucleus of the cell (Grote et al, 2004; Demirel et al, 2013).
Unfortunately, there is no clear agreement about water content in the nucleus of aged and cataractous lenses, with some analyses showing decreased water in the nucleus [ 50, 56- 59], some finding no change with aging or cataract state[ 46, 52] and yet others showing an increase in nuclear water content with aging[ 60] and cataract [ 61].
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EA treatment significantly reduced the nuclear p65 content, accompanied by a decreased ratio of p65 protein content in the nucleus to that in the cytoplasm in the adipose tissue.
To do this, we measured the DNA content in the nuclei of each callus using flow cytometry.
Moreover, Cyclin D1 content in the nuclei of Ts65Dn progenitors diminished to similar extents as in Tg Dyrk1a progenitors (Figs. 1C and 7B).
The general decrease observed in the signal intensity within the intervertebral disc (not the "SM" parameter limited to the nucleus) with increasing the severity of scoliosis could be related to the lower glycosaminoglycan content and increased collagen content in the nucleus pulposus of scoliotic discs observed by Pedrini et al [ 5] and Zaleske et al. [ 6].
Makino S, Gold PW, Schulkin J. Effects of corticosterone on CRH mRNA and content in the bed nucleus of the stria terminalis; comparison with the effects in the central nucleus of the amygdala and the paraventricular nucleus of the hypothalamus.
Collectively, these results indicate that the acetylation of G1 and K2 residues in γD-crystallin likely induced a molten globule-like structure, predisposing it to aggregation, which may account for the high content of aggregated proteins in the nucleus of aged and cataractous human lenses.
During the degeneration of intervertebral disc in humans, reduction of water content in the nucleus pulposus by MR imaging precedes a decrease of disc height as shown by X-ray [ 30, 31].
With idiopathic scoliosis, the intervertebral discs become wedged and narrowed, due in part to altered biomechanical environment [ 3]. Biochemical changes were also observed, such as lower glycosaminoglycan content, increased collagen content or increased total protein content in the nucleus pulposus or in the convex side of scoliotic intervertebral discs [ 4- 8].
Chromosomes are found in the nucleus of cells.
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