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Fura2 Ca2+ imaging experiments were performed on a BD Pathway 855 High Content Imaging System (BD Biosciences, Heidelberg, Germany).
The cells were then washed twice in PBS and 15 images collected per well on an Operetta high content imaging system (Perkin Elmer).
Quantification of the percent of nuclei surrounded by MHC-staining was achieved using the high content imaging capabilities and analysis software of the BD Pathway.
We report a cell-based assay using high content imaging to quantify myoblast differentiation by the presence of MHC expression surrounding each nucleus in the field.
Image acquisition was done using Nikon TI eclipse high content imaging enabled microscope running NIS elements high content imaging software (version 4.30.02).
Fluorecent images were taken using an Operetta High Content Imaging System instrument (PerkinElmer; Waltham, MA).
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The goal of this study was to develop and characterize high-magnification high-content imaging and image analysis methods suitable for high-throughput compound screening.
Plates were imaged on an ImagXpress Micro automated high-content imaging system (Molecular Devices), set to capture 3 sites per well, at 10× magnification.
Cell plates were then imaged on a BD pathway 855 high-content imaging system (BD Biosciences) for live-cell tracking studies with environmental control (37°C, 5% CO2).
Cells were imaged at 10× magnification in an IncuCyte Zoom Live-content imaging system (Essen Bioscience) at 37°C, 5% CO2.
were characterized using further immunofluorescence and the microarray scanner-based high-content imaging technique we developed previously[6, 8].
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