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Antineutrino detection using inverse beta-decay conversion has demonstrated capability to measure nuclear reactor power and fissile material content for nuclear safeguards.
The polymorphic content for nuclear and mitochondrial primers were analyzed using Bionumerics software [ 38].
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It remains to be seen if DNA strand asymmetry can also affect the composition of proteins encoded by eukaryotic nuclear genes, as has been shown for biases in the GC content of nuclear genes.
Then, supernatant containing nuclear extract was collected to evaluate the content of nuclear proteins.
To determine the ploidy and confirm the haploid form, flow cytometry for nuclear content was conducted.
Characterisation of thin titanium films was performed ex-situ by Rutherford backscattering spectroscopy (RBS) for Ti content and nuclear reaction analysis (NRA) for oxygen contamination.
After fixation, the cells were incubated with RNase A (0.1 mg/mL) at 37°C for 30 min, stained with propidium iodide (50 µg/mL) for 30 min and then measured for nuclear DNA content using BD-LSR Flowcytometer (Becton–Dickinson, USA) equipped with electronic doublet discrimination capability using blue (488 nm) excitation from argon laser.
Intraspecific variation for nuclear DNA content has been reviewed by Greilhuber (2005).
The mtDNA copy number per cell was calculated using Sdha amplification as a reference for nuclear DNA content.
However, assignment of estimated nuclear DNA contents to specific C-values in the present study is presumptive in that no karyological investigations were conducted on the algal samples used for nuclear DNA content estimates.
Assignment of estimated nuclear DNA contents to specific C-values in the present study is presumptive in that no karyological investigations were conducted on the algal samples used for nuclear DNA content estimates.
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