Because cellular contents concentrated toward Lo phase of the membrane, an increase in Ld phase due to incorporation of polyunsaturated linoleic acid separated a single cell into two distinctive poles with one rich and the other poor in cellular contents.
The total carbon, SOC and TN content were concentrated on the 0 4 cm depth, accounting for more than 50% of the total content.
The content was concentrated to 1 μL for gas chromatography analysis, and 1 μL was injected into the injection port of gas chromatography (GC).
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After 250 mL of water had passed, the cell content was concentrated in vacuo and subjected to preparative HPLC (column: YMC C18 ec 250 × 30 mm; detection wavelength, 220 nm; eluent A, MeCN with 0.1% TFA; eluent B, water with 0.1% TFA; gradient 25%to50%0% B in 20 min, t R(dodecaethyl-TRAP MDP 3) dodecaethyl-TRAP MDP 3hyl-TRAP(PDP)3) = 12 min).
Each total RNA extract was first treated with RNase-free DNase I (TaKaRa, Dalian, China) to remove contaminating DNA, and the mRNA content was concentrated by capturing on magnetic oligo (dT) beads.
These findings are congruent with conclusions drawn in Watanabe et al. (2002) [ 29], where it was shown that early/late transitions in replication timing correlate with high SNP frequency, increased DNA damage, transitions in GC content, and concentrated occurrence of disease-related genes.
The dispersed contents were concentrated by evaporating the ethanol in a rotary evaporator.
Comparisons between those widely divergent genomes revealed that most of the differences in GC-content are concentrated in external gene regions, upstream first or downstream last intron.
For each detergent, ∼20 μL of eluted protein was diluted to 500 μL with exchange buffer (see Protein Expression and Batch Purification section for buffer contents) and concentrated to ∼100 μL using a 3.5 kDa spin column (Millipore , Inc.
