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We determined the DNA content by quantitative PCR for the single-copy gene β-globin and total RNA content using quantitative image analysis of ribosomal bands.
We also elucidated total fat content by quantitative micro-CT analysis.
Fractions were then analyzed for Ad5 genome content by quantitative polymerase chain reaction (PCR).
In this subset, we also measured telomeric DNA content by quantitative polymerase chain reaction (qPCR) analysis, which strictly quantifies telomere repeats.
Blood samples (20 μL) were taken at defined time points and immediately separated into cell and plasma fractions; and after washing of the cell fraction in PBS, both fractions were assayed for Ad5 genome content by quantitative PCR.
This was accompanied by a 45% reduction in e17.5 placental mtDNA content by quantitative PCR (qPCR; Fig. 2C; supplementary material Table S4), associated with a trend towards decreased protein levels of COX1, a mtDNA-encoded subunit of cytochrome c oxidase (Fig. 2D).
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VLP preparations were titrated by measuring the HIV-1 contentsntents by quantitative ELISA (Abbott, Abbott Park, Illinois), and by the reverse transcriptase assay [ 38].
Although DNA copy number will often differ between species, and primer efficiency will differ among DNA sequences, the number of DNA molecules detected from faeces or digestive tract contents by quantitative PCR can be used as a semi-quantitative measure of prey biomass [ 79].
Seventeen patients with small bowel transplants were recently studied over a period of 8 weeks and they had their ileal content measured by quantitative PCR [ 52].
GFP-expressing plasmids containing either wild-type or the 3′UTR variant cDNA were transfected into RT112M cells, and protein expression assayed by FACS and mRNA content assayed by quantitative real-time reverse transcription PCR.
Activity was normalized to CK-B content by performing quantitative western blotting in the same samples.
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