Therefore, in order to accurately estimate the hydrolysis yield in this study, we measured the WIS content and analyzed the solid composition according to standard NREL procedure (Sluiter et al. [2008b]) after each fermentation.
Cell lysates were prepared at the end of the incubation, standardized by protein content, and analyzed by western blot for the expression of HSP60.
Cell lysates were then collected, normalized by total protein content and analyzed under reducing conditions with 5× SDS-gel loading buffer (50 mM Tris-HCl pH 6.8; 100 mM dithiothreitol; 2% (w/v) electrophoresis grade SDS; 0.1% bromophenol blue; 10% (v/v) glycerol on a 10% SDS-PAGE.
For the purposes of data analyses, questions were categorized by content and analyzed by category.
Akira Kanesaki critically revised the article for important intellectual content and analyzed and interpreted the data.
LI and ML developed the theoretical framework, elaborated the research hypotheses, devised the methodological content, and analyzed the data.
