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The aphids feed from the phloem sieve element content and analyses of this sap in selected maize lines revealed marginally, but significantly higher amino acid levels in Bt maize, which might partially explain the observed increased aphid performance.
They were then washed and resuspended in propidium iodide/Triton X-100 staining solution containing RNase A. Flow cytometry was used to detect DNA content, and analyses of cell distribution in the different cell cycle phases were performed using FlowJo 7.5 (Tree Star, Inc., Ashland, OR, USA).
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At 10 h after treatment, cells were stained with propidium iodide (PI) to detect DNA content and analysed by flow cytometry.
To estimate these models, we divided each coverage profile according to the respective GC content and analysed the corresponding data separately.
We adjusted our results for GC content by splitting each coverage profile into separate data sets comprising the coverage values of windows with similar GC content and analysing them separately [ 4, 5].
The interviews were all transcribed verbatim, coded and analysed (content and discourse analyses) with the help of qualitative computer software (NVivo) [ 34], including simple counting [ 35].
Data were analysed using content and thematic analyses.
Content and thematic analyses were used to analyse the interview transcripts.
The programme's content, delivery and analyses were consistent with SDT hypotheses [9].
The data is typical of extant genome content, annotation, and analyses.
Four plants of each genotype were harvested at 0%, 50%, and 70% water loss for biomass, water content, and hormone analyses.
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