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The main advantages of this approach are that 1) we account for all pair-wise correlations between variables simultaneously and 2) we correct for possibly confounding factors, such as GC content, alignment length, and alignment depth.
In this study, we have performed RIPCAL analyses using 3 different models: highest G C content, alignment majority consensus and predicted sequence of the repeat family prior to RIP-mutation.
RIP dominance [ 20] was calculated using a variety of comparative models including: sequence of highest G C content; alignment majority consensus and; consensus sequence predicted by deRIP (Additional file 5).
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Because of this low neutral sequence conservation and the extreme AT content, alignments of the four species are less reliable except in regions with strong conservation.
Analyses of the whole-genome alignments reveal significant associations between support for the accepted species relationship and specific characteristics of the genomic regions, such as GC-content, alignment score, exon content, and conservation.
Modification types are inferred from BLAST alignments of input sequences to their hairpin precursors and then examining the content of alignment mismatches returned.
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Qualitative and quantitative analysis of the data showed significant differences between affected and lesion-free areas in terms of collagen content, fibril alignment, and total tissue volume.
Based on MWCNT content, the alignment of nanotubes contributes to reduced collisions among which affects the penetration threshold significantly.
That information then gets plugged into an algorithm to flood you with content in alignment with your perceived beliefs, skewing your perception of the world.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com