Regardless of strategy, the massive P. taeda genome will surely challenge the limits of contemporary sequencing technology.
Contemporary sequencing technology can sample genomes comprehensively in only hours, with these data SNP detection is typically achieved by aligning reads to a reference genome and identifying SNPs as a difference between consensus and the reference (Leggett and MacLean, 2014).
Abbreviations: S, segregating sites; θ, Watterson's theta; π, Tajima's pi; D, Tajima's D. While contemporary high-throughput sequencing technologies make genome-wide studies feasible in both model and non-model species, whole-genome re-sequencing is usually still cost prohibitive.
The rapid advances in sequencing technologies have facilitated the development of comparative genomics – an important approach in contemporary evolutionary biology research [ 1, 2].
A handful of next-generation sequencing technologies are now being developed and constantly improved each year.
NGS is something of a catch-all term describing a variety of sequencing technologies that have largely replaced Sanger sequencing.
How much do the sequencing technologies cost?
New sequencing technologies now make this possible.
Sequencing technologies keep evolving toward completing genomes.
DNA sequencing technologies have improved rapidly.
