Sentence examples for contamination with RNases from inspiring English sources

Standard precautions were undertaken to avoid contamination with RNases during the purification of total RNA [43].

In addition to the issues discussed, it is of obvious importance to stringently control contamination with RNases.

A contamination of the T7 RNA Polymerase with RNases would seem plausible, yet three arguments contradict this: (i) Commercial T7 RNA preparations are usually highly purified and tested for contamination with RNases.

Special care was taken to avoid contamination with RNase, and to avoid cross-contamination between reactions.

To avoid degeneration, cancer tissue centres and corresponding cancer-free tissues were snap frozen in liquid nitrogen immediately after excision, handled carefully to avoid contamination with RNase, and stored at −80°C until use.

To eliminate contamination with exogenous RNases, water and solutions such as PBS and sheath fluid, were treated with diethylpyrocarbonate (DEPC; 0.1% followed by autoclaving).

CAUTION: When working with RNA always use clean gloves, RNase-free plasticware and barrier pipette tips to prevent contamination of samples with RNases.

DNA contamination was removed with RNase-Free DNase Set (Qiagen).

Genomic DNA contamination was removed with RNase-free DNase I (TaKaRa, Japan) according to the manufacturer's instructions.

Qualified total RNA was further purified with RNeasy micro kit (QIAGEN, GmBH, Germany), and genomic contamination was removed with RNase-Free DNase Set (QIAGEN, GmBH, Germany).

Cuvettes and stir bars were soaked in HCl for 15 min to eliminate RNase contamination, thoroughly rinsed with RNase-free water, and then blocked for 1 h with 250 mM KCl, 10 mM potassium phosphate (pH 8), 1 mM MgCl2, and 40 μg/mL BSA.