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A potential limitation of our analysis is the lack of environmental mold sampling; however, whether this would be contributory or as valuable as wound contamination sampling is not clear.
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This can be assessed in terms of the concordance between the contamination sample and observations identified as having elevated Wi.
The deionized water was considered as a possible source and, to establish the extent of contamination, samples were procured from universities in Italy, the U.S.A. and England.
The investigation also identified the source of the contamination: Samples from the nearby Stewart Air National Guard Base tested for high levels of PFOS.
As a control for cross contamination samples consisting of distilled water were also subjected to the isolation method and the extracts were tested with all assays.
To avoid blood contamination, samples were not collected when oral disease, inflammation, or lesions were present.
In case of DNA contamination samples were manually re-digested by DNase I treatment.
In the case of DNA contamination, samples were manually re-digested with DNase I.
In collection stage can occur: contamination, sample evaporation and inadequate time in the sweat production.
To prevent contamination, samples were put in sterile cryo tubes and then placed in liquid nitrogen.
To eliminate any DNA contamination, samples were DNase I treated using DNA-free™ (Ambion).
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