The source of C. difficile in retail meats may involve antemortem deposition of spores in the animal's muscle or other tissues, fecal or environmental contamination of carcasses, or contamination during processing.
This allows their digestive tracts to empty and reduces the potential for contamination during processing.
An exhaust vent leading from one of the facility's "clean rooms" to the roof lacked a filter or screen, for example, and flaws in the air handling system could expose sterile drugs to microbial contamination during processing.
Chromium content of food varies widely due to differences in soil mineral content, growing season, plant cultivar, and contamination during processing.
Taking these results together, the most plausible explanation is that the no γ-decalactone pools had some contamination during processing with some fruits containing the volatile.
The molecular methods used in our study have been previously validated (7, 8 ), and precautions were taken to reduce risks for contamination during processing.
Such a very high positive result in their study is likely due to either geographical/ethnic/racial differences or potential contamination in processing.
Another reason for the observed high thermal stability may be impurities which are introduced in the latter bulk compacts from the as-received powders or from contamination during processing.
1 µg total cellular RNA was digested with 1 unit DNase I (Promega) at 37°C for 20 min to remove genomic DNA contamination, before processing for reverse transcription (RT) with oligo dT) primers and reverse transcriptase of Moloney murine leukaemia virus (Promega).
In addition, their cross-contamination in processing stages is irrefutable.
