In order to define a starting condition, the test plates were analyzed using the BonNDTinspect system before every contamination procedure.
The panel concluded that using the surrogate infection model to demonstrate efficacy has a sound scientific basis, that the use of Shigella flexneri as a test organism coupled with a modified hand contamination procedure is supported by published data, and that the model represents a realistic test for the efficacy of consumer antibacterial handwash products.
After sieving, the titanium particles were subjected to standardized cleaning to remove potential particulates and chemical contamination (Procedure Number PS03-016; CAM ImpLeidenBV, Leiden, The Netherlands).
This cycle was followed by a 30-second handwash using nonmedicated soap, a 30-second rinse and a second contamination procedure.
Additionally, the low-volume contamination procedure is simpler to execute and more closely mimics the condition of hands when ABHR use is indicated in clinical settings (e.g. minimally soiled and fully dry).
For plant interactions, maize seeds (Silver Queen hybrid) were treated with 70% ethanol for 5 min, rinsed with water, soaked in 10% hydrogen peroxide solution for 2 hours, rinsed with water, and germinated on moist sterile filter papers to screen for contamination (procedure modified from: Djonovic et al., [ 17]).
"This technique is open to the same risk-assessment and anti-contamination procedures".
The ABC is physically isolated, subject to stringent anti-contamination procedures, equipped with positive air pressure and UV lighting, and has a DNA laboratory and equipment (glove box, instruments, full body suits, protective masks, etc).
Also all other common anti-contamination procedures were followed to avoid any potential risk of contamination.
The failure of Cadbury to inform the Food Standards Agency for five months after the problem was discovered resulted in a £1 million fine from Birmingham's Crown Court, and the Food Standards Agency advised the company to improve their "out of date" contamination testing procedures.
