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While this analysis does not rule out the possibility of partial contamination of the array results by some weak cross-hybridization, to guard against this possibility we carefully designed probes to be species-specific under the stringent hybridization conditions used in this study.
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Two explanations are (1) that a paralogous gene is undergoing change in expression giving cross-hybridization on the array but not giving PCR product because of the greater stringency of the RT-qPCR and (2) that there is contamination of the arrayed spots attributable to splash during transfers to the microtiter plates or carry-over of the array pins.
A tissue microdissection technique was used to reduce the contamination of the normal tissue components for the array CGH analyses, as previously described [ 10].
However, due to contamination buildup, which is more substantial toward the edges of the array, we no longer recommend that the nominal aimpoint be shifted from the center of the detector.
(b) An assessment of the adapter contamination of the sample.
Conversely, symbiont contamination of the host tissue was negligible.
What caused this alarming contamination of the White House lawn?
Oil contamination of the microcosms was then performed (2% v/wt contamination).
Bacterial contamination of the stethoscope was significant.
This prevents contamination of the products.
This indicates that the observed microarray results from the HS experiment cannot be explained as the result of contamination among probes on the array.
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