The husbandry protocols should also be revised so as to minimize accidental cross-contamination of lines.
The remaining 51 DH lines were excluded based on examination of the molecular marker data: 43 because they seemed to be genetically identical to other lines in the population, 6 because the DNA samples representing those lines exhibited more than one allele for numerous markers, indicating possible contamination of the lines or DNA samples, and 2 due to possible errors in DNA extraction.
Also in December, a RIKEN investigative team concluded that STAP cells never existed and that indications of pluripotency reported in experiments likely resulted from contamination of cell lines.
Thus, we conclude that the low temperatures obtained by our FPIs for low airglow intensities are probably due to the contamination of OH lines, particularly due to the overlapping of the OH (630.68 nm) fringes on the OI (630.038 nm) fringes.
One other consideration is the propensity for viral contamination of PDX lines, particularly with lactate dehydrogenase-elevating virus.
The most common causes of cell misidentification include mislabeling, contamination of cell lines (i.e. feeder cells used in cocultivation of human stem cells), and xenografting (transplanting living cells, tissues, or organs from one species to another) [ 7, 8].
However, we found that temperatures below 400 K were obtained associated with weak airglow intensities and concluded using a model calculation that they are due to contamination of OH line emissions in the upper mesosphere.
General Mills has installed doors in all its equipment so that it can more easily be inspected for stray ingredients; in Hershey's plants, some manufacturing lines are reserved for nut products and nut products alone, to avoid cross-contamination of other lines.
STR analysis allowed to exclude possible cross contamination of analysed cell lines excluding H-318 (murine cell line).
Cross-contamination of cell lines with HeLa cells was first reported in 1968 [39].
Because of the increasing importance of cultured cells in cancer research and other biomedical studies, cross-contamination of cell lines is becoming a more frequently encountered problem.
