A third strength of in situ RT-PCR relates to the issue of sample contamination in solution-phase PCR.
Standard precautions were taken to eliminate ribonuclease contamination in solutions and glassware including baking all glassware at 200°C for six hours, and treating all solutions (except those containing Tris) with 0.1% diethylpyrocarbonate for 12 hours followed by autoclaving.
Hundreds of thousands of horseshoe crabs are harvested every year by the medical community, because the crucial chemical in their blood can detect contamination in any solution that might come into contact with blood.
The MS/MS measurement also revealed that the signal at m/ z 511.3, which appeared also in the full-scan mass spectrum (m/ z 511.2), was only caused by in-source fragmentation and was not due to another compound (or contamination) in the solution.
(A) Filtration could decrease the miRNA contamination in AMV solutions.
As shown in Fig. 2A, filtration could decrease the miRNA contamination in AMV solutions: all the CT values of four miRNAs were decreased about 1 2 cycles, which indicates that the miRNAs in AMV solution might be separated from the reverse transcriptase in this condition.
The starting levels of Zn contamination in physiological solutions relies on the purity of the deionized water and chemicals.
To explore the role of zinc (Zn) in cellular physiology it is important to be able to control and quantify the level of Zn contamination in experimental solutions.
The frequency of bacterial contamination found in solutions for surface disinfection based on surface active ingredients was surprisingly high with an overall rate of 42%.
Probably, these microbubbles are associated with water molecules desorbed from inner layers of the GO film as well as originated to uncontrolled contaminations in liquid solution of GO.
Several possible reasons for experimental failure were brought up in the first part of this paper, where various aspects that are not always taken into account were underlined (possible experimental mistakes, poor washing step, evolution of a substrate in solution, contamination of a medium by microorganisms, residual presence of precursor phases, etc).
