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While it was straightforward to separate main body from sub-mantle tissue with little cross-contamination, collection of only cement gland tissue within the sub-mantle cavity proved more challenging.
To address these issues, we first evaluated the potential for contamination from collection and analytic materials and identified standard collection materials, water and analytical apparatuses that are free from BPA contamination.
A casual (or spot) urine specimen was collected from the participants after confirmation of no background contamination in collection materials (NCHS 2004b).
The samples were collected when the study subjects were hospitalized, which reduced the risk of contamination and collection errors.
Phthalate data were collected in NHANES as urinary metabolites rather than unmetabolized phthalates in serum to eliminate contamination during collection and analysis (Latini 2005).
This effort required extraordinary precautions to avoid particle contamination during collection and in the laboratory.
Laboratories screened materials to identify BPA contamination in collection and analysis materials.
Until recently, positive blood cultures for CoNS were regarded as contamination during collection of the sample.
To minimise the risk of contamination, the collection of the specimens was performed at different days using disposable material.
To minimize contamination, data collection from DUHS medical college was completed in 2 days and from AKU medical college over the subsequent two days.
Detailed BPA analysis methods have been published, including the quality control system that was used to prevent contamination during collection, handling, and analysis of samples (CDC 2007b).
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