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The use of MS with alternately clean and contaminated laminae clearly shows that differential contamination cells are capable of generating potential differences (driving forces) of hundreds of millivolt, as also occurred with underfilm differential aeration cells.
Background contamination (cells other than spermatozoa) was removed by gating.
Upon clearance of the contamination, cells again grew to confluence and formed an electrically confluent monolayer.
To remove any residual cytoplasmic extracts contamination, cells were washed using buffer A, followed by PMSF added PBS.
To avoid a possible fibroblast contamination, cells were cultured at passage one for three days with D-valine-substituted DMEM (Sigma-Aldrich, St Louis, MO, USA).
To minimize fibroblast contamination, cells were pre-seeded in a culture dish for 3 hours in F10/HAM, 20% FBS, 1% penicillin/streptomycin (PS), 1% Fungizone.
Similar(53)
All cell lines were tested for mycoplasma contamination, cell proliferation and morphology evaluation, both after towing and within four passages in culture.
To confirm the purity of the cell population and to exclude the presence of hematopoietic cell contamination, these cells should lack expression of CD3, CD14, CD19, CD34, CD45, and HLA-DR as determined by flow cytometry.
To remove mesenchymal cell contamination from carcinoma cells, we examined the expression of LMO4, LDB1, and LDB2 in 12 different squamous carcinoma cell lines.
However following a singular aspiration step with the above described preset conditions stem cell contamination with feeder cells was never observed.
To avoid normal cell contamination, target epithelial cells from the cancer areas were produced by laser capture microdissection using a Pixcell LCM system (Arcturus Engineering Inc., Mountain View, CA, USA).
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