Exact(2)
When the contamination amount and sample size decrease, (E_{k2}) gives better results.
In this study, using a 25−1 factorial fractional design, we first evaluated the effects of fermentation time, of initial sucrose concentration and of the initial contamination amount of a spoilage yeast, on antifungal activities of single and mixed cultures of Lactobacillus rhamnosus K.C8.3.1I and Lactobacillus harbinensis K.V9.3.1Np in yogurt.
Similar(58)
These tests are able to detect contamination amounts above 1-2%.
As a control for genomic DNA contamination, an equivalent amount of total RNA without reverse transcription was tested.
Such screening would help reduce the incidence of U.S. food contamination and the amount of contaminated consumed food.
However, simultaneously control of the severe contamination of large amount of chromium and chloride discharge, both of which are serious environmental concerns, is highly challenging with the conventional chrome tanning process.
As a control for plasmid contamination, an equivalent amount of virus was either boiled for 10 minutes before infecting cells or the reverse transcriptase inhibitor efavirenz (100 nM) was added at the time of infection.
In fact, the surface roughness may have a significant impact on the contamination [ 2], amount and quality of plaque formation [ 40].
The reason for that may be due to incomplete annotation, strong interaction with the proteins located at outer mitochondrial membrane, or contamination of trace amount of other organelles.
To minimize the contamination of trace amount of divalent metal ions, all "metal free" buffers and reagents were prepared using deionized water (Barnstead System) and further purified by and stored with Chelex® 100 (Sigma) before use.
To ensure no genomic DNA contamination, an isolated amount of 2 μg of each RNA sample was treated with DNase I (Fermentas, Lithuania) and directly used as a template for cDNA synthesis.
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