However, some contaminating proteins still remain in the eluate from the affinity column.
An efficient, single-step purification procedure eliminating all contaminating proteins including, importantly, the non-myristoylated matrix protein was designed.
Continuous size exclusion chromatography for the separation of cell culture-derived influenza virus from contaminating proteins was established successfully.
Then, the agarose was washed with a stepwise gradient of imidazole (10 40 mM) to eliminate intracellular contaminating proteins.
A variety of purification techniques including, ammonium sulfate precipitation, gel filtration and ion exchange chromatography are required to purify laccase from other pigments and contaminating proteins.
Introduction of a second purification step with Protein L successfully removed contaminating proteins and heavy chain fragments.
All proteins identified were associated with insect saliva; therefore the method was regarded as successfully generating saliva proteins in the absence of contaminating proteins from other sources.
Contaminating proteins and phospholipids were removed and the purified CPS was further subjected to proteinase K digestion, DNAse and RNAse treatment followed by extensive dialysis [19].
Three major limitations are usually considered in the interpretation of functional proteomics datasets - (i) fidelity of the protein identification; (ii) proteins missed; and (iii) "contaminating" proteins found.
Although our tandem purification procedure greatly reduces the presence of background proteins, we can still identify, on average, 2 6 contaminating proteins in the control samples.
During GST-Hp1018/19Δsp preparation, contaminating proteins were co-purified, which were identified by MALDI-TOF-MS as glutathione-S-transferase and degradation products of HtrA.
