Sentence examples for contaminating fibroblasts from inspiring English sources

However, previous studies often lack detailed isolation protocols or a precise characterisation of the culture composition excluding especially contaminating fibroblasts.

Thenafter, tissues were eliminated and contaminating fibroblasts were selectively killed by treating the cultures with 100 μg/ml G418 for 3 4 days.

After outgrowth, contaminating fibroblasts were removed using magnetic anti-fibroblast beads (Miltenyi, Bergisch Gladbach).

In a typical growing population, 30 to 40% of the proliferating cells do not express CD56 and are usually considered as "contaminating" fibroblasts [12].

The pellet was resuspended in 10 ml L15 + 10% FBS and plated in uncoated 100-mm Petri dishes for 20 30 min depending on the amount of tissue, to allow contaminating fibroblasts to settle out.

Satellite cell and myoblast cultures were prepared from whole muscles by isolating mononuclear cells from 2-day old neonates and performing pre-plating steps to remove contaminating fibroblasts or by FACS sorting as described [25], [55], [56], [57].

The medium is supplemented with 20 μM of cytosine-arabinoside (Cell Pharm GmbH, Hannover, Germany) to suppress the growth of contaminating fibroblast-like cells.

Contaminating cardiac fibroblasts were removed by pre-plating cells for 2 hours in uncoated culture flasks.

The adherent component of the cultures, containing hMSCs is relatively pure, but frequently contains traces of contaminating osteoblasts, fibroblasts and senescent cells.

Cell counts were performed on permeabilised, fixed samples stained with cardiac specific markers so rule out increases in cell number by contaminating cardiac fibroblast cells.

While it is possible that the observed iCMs were actually remote cardiac progenitor cells contaminating the fibroblast population, this is unlikely for two reasons.