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In previous studies in Finland and Germany, the occurrence of legionellae was similar (30% and 26%, respectively) as well as the contaminating concentration (20, 21 ).
However, taking into account the contaminating concentration of Cu measured in HMA6 phosphorylation assays, HMA8 phosphorylation is maximal at a lower Cu+ concentration than that of HMA6, suggesting that HMA8 would have a higher apparent affinity for Cu+ than HMA6.
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However, contaminating calcium concentration was 21±1.3 µM, as was determined with inductively coupled plasma optical emission spectroscopy (ICP-OES).
Differences between cycle threshold (Ct) values of purified RNA samples in presence and absence of RT were typically > 20, corresponding to a 40-fold difference between RNA over contaminating DNA concentration, confirming the quality of RNA isolates.
The apparent ability of Mn2+ to promote the formation of ROS within a cortical mitochondrial-synaptosomal fraction was quenched by the depletion of contaminating nanomolar concentrations of trivalent metals.
RNA and contaminating DNA concentrations were determined using a Qubit fluorimeter (Life Technologies, Paisley, United Kingdom).
Even if the dredged material is only slightly contaminated, bioavailability, concentration in the food chain as well as long-term and combined effects of contaminants may still be an issue [42].
As discussed below, the IC50 of phosphopeptide with t-PTEN is ∼1 μM and the contaminating phosphopeptide final concentration in 4p-PTEN contassays assays is estimated to be less than 10 nM.
Unlike OTCs, chemical analyses of PAHs showed that sewage effluents were the major source of contamination as sites located downstream from the discharge of sewage effluents were heavily contaminated (PAH concentrations ranged from 172 to 10,188 ng/g dw).
The U.S. EPA (1998) considers a surface to be contaminated if concentrations exceed 10 μg/100 cm.
A total of 7 ferries WDS were contaminated with concentrations of ≥104 CFU/L in at least one sample, whereas 4 of 7 ferries were colonized with ≥105 CFU/L in at least one sample.
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