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Therefore, even very low numbers of contaminating cells will produce significant contamination.
Although many of these contaminating cells may be removed during passaging, such contamination would affect the expansion of MSCs as well as the overall effect of the therapy.
An average 95-fold decrease in leukocyte contamination was seen for samples containing high levels of contaminating cells.
Supplementary Figure 5 Characterization of contaminating cells in hESC derived EN cultures.
Consequently, it is not always clear whether DFAT cells are derived from dedifferentiated mature (lipid filled) adipocytes or from contaminating cells that reside in an impure culture.
In purifying the granule cells, what the scientists first believed to be contaminating cells turned out, surprisingly, to be stem cells.
Exclusion of these contaminating cells did not alter the differential sphere forming activity (Figure 5).
The contaminating cells in our cardiomyocyte cultures consisted predominantly of cardiac fibroblasts (>95%, data not shown).
Supernatants were carefully transferred to microfuge tubes, centrifuged to remove contaminating cells and stored at −80°C until use.
Due to the moderate purity of CliniMACS isolated Treg populations, outgrowth of contaminating cells could be a risk upon expansion.
The faint bands observed in ΔTrsp keratinocytes were most certainly due to contaminating cells as further discussed below.
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