Sentence examples for contaminating activity from inspiring English sources
This enzyme was originally detected as a contaminating activity when highly purified preparations of dipeptidylpeptidase I (DPP I) were employed in time-course studies designed to establish the extent of dipeptide release from polypeptide hormones, such as pig α-corticotropin (ACTH) and glucagon.
As this is not observed for the Tg single-stranded substrate, the possible contaminating activity might be sequence dependent.
More stringent purification and equipment cleaning procedures as well as routine testing for contaminating activity have been put in place at PBL in part due to these experiments.
Moreover, additional experiments must usually be run to determine the particular activity of interest in the co-incubated cell lines themselves to determine contaminating activity.
Catalytic properties of these two gene products have been analyzed extensively (K. Gonzales-Rivera, M. Jimenez-Rodriguez), with attention to likely sources of contaminating activity.
Contaminating activity from a tiny amount of wild type full-length enzyme 10-fold more active than the Urzymes would exhibit an insignificant burst, and the entire time course would represent its steady-state rate.
Importantly, ubiquitination was strictly dependent on the Vpu-SCF complex, arguing against any other contaminating activities in the system that could explain the lack of difference.
NEIL1 G83D was purified to apparent homogeneity (supplementary Figure 1 is available at Carcinogenesis Online), tested for co-purifying contaminating activities (supplementary Figure 2 is available at Carcinogenesis Online) and assayed toward several oxidative DNA lesions known to be removed by NEIL1.
The residues mutated in these proteins are known to be essential for ATP binding and hydrolysis [6], [13], which allowed us to include these loss-of-function mutants as controls to rule out the presence of any possible contaminating ATPase activity, as well as ATP-independent DNA unwinding activities.
In all tests, contaminating ribonuclease activity of plastic ware, reagents and buffers was excluded.
Velocity gradient purification resulted in a reduction in a contaminating RNase activity (Fig. 3).
